Tags

• Fast relaxation imaging 1
• Protein Folding 1
• Fluorescence 2
• single-molecule 3
• Passivation 1
• DDS 1
• smFRET 4
• Super_Res 2
• STORM 2
• In Situ Kinetics 2
• Bacterial Small RNA 2
• CRISPR 5
• Single-molecule 6
• Dual-mode binding of Cas9 1
• Cas9 6
• gene editing 4
• Kinetics 1
• Centroid-localization 1
• CRISPR Review 1
• EngCas9 2
• Cas12a 1
• Genome-Engineering 1
• Cpf1 1
• Non Canonical gRNA 1
• cryo-ET 1
• In situ tomography 2
• cryo-FIB-ET 1
• cryo-FIB 1
• Aquilos 1
• Cryo-EM 1
• Milling 1
• Tomography 1
• CRISPR-Cas9 1
• conformational rearrangement 1
• single molecule 1
• Phase-separation 1
• Cryo-tomography 1
• COVID-19 1
• Droplets 2
• SARS-CoV-2 1
• Viral-assembly 3
• Phase separation 1
• ALS 1
• Neurodegeneration 1
• TDP-43 1
• cryo-Tomography 1
• grants 1
• Motor 2
• ATPase 2
• Genome-Packaging 2
• Nuclear Transport 2
• Nuclear Pore Complex 2
• Integrative Modeling 2
• Nuclear Basket 2
• Cryo-electron tomography 2
• nucleocytoplasmic transport 2
• cryo-focused ion beam milling 3
• Subtomogram Analysis 3
• mRNA transport 1
• Chromatin Organization 1
• In-cell cryo-electron microscopy 1

Fast relaxation imaging

• Protein stability and folding kinetics in the nucleus and endoplasmic reticulum of eucaryotic cells

Protein Folding

• Protein stability and folding kinetics in the nucleus and endoplasmic reticulum of eucaryotic cells

Fluorescence

• An Improved Surface Passivation Method for Single-Molecule Studies
• Protein stability and folding kinetics in the nucleus and endoplasmic reticulum of eucaryotic cells

single-molecule

• Single molecule analysis of effects of non-canonical guide RNAs and specificity-enhancing mutations on Cas9-induced DNA unwinding
• The Single-Molecule Centroid Localization Algorithm Improves the Accuracy of Fluorescence Binding Assays
• An Improved Surface Passivation Method for Single-Molecule Studies

Passivation

• An Improved Surface Passivation Method for Single-Molecule Studies

DDS

• An Improved Surface Passivation Method for Single-Molecule Studies

smFRET

• Single molecule analysis of effects of non-canonical guide RNAs and specificity-enhancing mutations on Cas9-induced DNA unwinding
• Mechanisms of improved specificity of engineered Cas9s revealed by single-molecule FRET analysis
• Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
• An Improved Surface Passivation Method for Single-Molecule Studies

Super_Res

• Effects of individual base-pairs on in vivo target search and destruction kinetics of bacterial small RNA
• Determination of in vivo target search kinetics of regulatory noncoding RNA

STORM

• Effects of individual base-pairs on in vivo target search and destruction kinetics of bacterial small RNA
• Determination of in vivo target search kinetics of regulatory noncoding RNA

In Situ Kinetics

• Effects of individual base-pairs on in vivo target search and destruction kinetics of bacterial small RNA
• Determination of in vivo target search kinetics of regulatory noncoding RNA

Bacterial Small RNA

• Effects of individual base-pairs on in vivo target search and destruction kinetics of bacterial small RNA
• Determination of in vivo target search kinetics of regulatory noncoding RNA

CRISPR

• Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a)
• Mechanisms of improved specificity of engineered Cas9s revealed by single-molecule FRET analysis
• Understanding the Molecular Mechanisms of the CRISPR Toolbox Using Single Molecule Approaches
• The Single-Molecule Centroid Localization Algorithm Improves the Accuracy of Fluorescence Binding Assays
• Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9

Single-molecule

• Comprehensive structure and functional adaptations of the yeast nuclear pore complex
• A viral genome packaging ring-ATPase is a flexibly coordinated pentamer
• Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a)
• Mechanisms of improved specificity of engineered Cas9s revealed by single-molecule FRET analysis
• Understanding the Molecular Mechanisms of the CRISPR Toolbox Using Single Molecule Approaches
• Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9

Dual-mode binding of Cas9

• Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9

Cas9

• Real-time observation of Cas9 postcatalytic domain motions
• Single molecule analysis of effects of non-canonical guide RNAs and specificity-enhancing mutations on Cas9-induced DNA unwinding
• Mechanisms of improved specificity of engineered Cas9s revealed by single-molecule FRET analysis
• Understanding the Molecular Mechanisms of the CRISPR Toolbox Using Single Molecule Approaches
• The Single-Molecule Centroid Localization Algorithm Improves the Accuracy of Fluorescence Binding Assays
• Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9

gene editing

• Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a)
• Mechanisms of improved specificity of engineered Cas9s revealed by single-molecule FRET analysis
• Understanding the Molecular Mechanisms of the CRISPR Toolbox Using Single Molecule Approaches
• Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9

Kinetics

• The Single-Molecule Centroid Localization Algorithm Improves the Accuracy of Fluorescence Binding Assays

Centroid-localization

• The Single-Molecule Centroid Localization Algorithm Improves the Accuracy of Fluorescence Binding Assays

CRISPR Review

• Understanding the Molecular Mechanisms of the CRISPR Toolbox Using Single Molecule Approaches

EngCas9

• Single molecule analysis of effects of non-canonical guide RNAs and specificity-enhancing mutations on Cas9-induced DNA unwinding
• Mechanisms of improved specificity of engineered Cas9s revealed by single-molecule FRET analysis

Cas12a

• Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a)

Genome-Engineering

• Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a)

Cpf1

• Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a)

Non Canonical gRNA

• Single molecule analysis of effects of non-canonical guide RNAs and specificity-enhancing mutations on Cas9-induced DNA unwinding

cryo-ET

• Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

In situ tomography

• HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells
• Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

cryo-FIB-ET

• Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

cryo-FIB

• Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

Aquilos

• Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

Cryo-EM

• Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

Milling

• Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

Tomography

• Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

CRISPR-Cas9

• Real-time observation of Cas9 postcatalytic domain motions

conformational rearrangement

• Real-time observation of Cas9 postcatalytic domain motions

single molecule

• Real-time observation of Cas9 postcatalytic domain motions

Phase-separation

• The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

Cryo-tomography

• The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

COVID-19

• The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

Droplets

• HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells
• The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

SARS-CoV-2

• The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

Viral-assembly

• Comprehensive structure and functional adaptations of the yeast nuclear pore complex
• A viral genome packaging ring-ATPase is a flexibly coordinated pentamer
• The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

Phase separation

• HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells

ALS

• HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells

Neurodegeneration

• HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells

TDP-43

• HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells

cryo-Tomography

• HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells

grants

• Proposal for Parenthood accepted

Motor

• Comprehensive structure and functional adaptations of the yeast nuclear pore complex
• A viral genome packaging ring-ATPase is a flexibly coordinated pentamer

ATPase

• Comprehensive structure and functional adaptations of the yeast nuclear pore complex
• A viral genome packaging ring-ATPase is a flexibly coordinated pentamer

Genome-Packaging

• Comprehensive structure and functional adaptations of the yeast nuclear pore complex
• A viral genome packaging ring-ATPase is a flexibly coordinated pentamer

Nuclear Transport

• The Molecular Architecture of the Nuclear Basket
• Integrative spatiotemporal map of nucleocytoplasmic transport

Nuclear Pore Complex

• The Molecular Architecture of the Nuclear Basket
• Integrative spatiotemporal map of nucleocytoplasmic transport

Integrative Modeling

• The Molecular Architecture of the Nuclear Basket
• Integrative spatiotemporal map of nucleocytoplasmic transport

Nuclear Basket

• The Molecular Architecture of the Nuclear Basket
• Integrative spatiotemporal map of nucleocytoplasmic transport

Cryo-electron tomography

• The Molecular Architecture of the Nuclear Basket
• Integrative spatiotemporal map of nucleocytoplasmic transport

nucleocytoplasmic transport

• The Molecular Architecture of the Nuclear Basket
• Integrative spatiotemporal map of nucleocytoplasmic transport

cryo-focused ion beam milling

• Cryo-Focused Ion Beam Milling of Cells
• The Molecular Architecture of the Nuclear Basket
• Integrative spatiotemporal map of nucleocytoplasmic transport

Subtomogram Analysis

• Cryo-Focused Ion Beam Milling of Cells
• The Molecular Architecture of the Nuclear Basket
• Integrative spatiotemporal map of nucleocytoplasmic transport

mRNA transport

• The Molecular Architecture of the Nuclear Basket

Chromatin Organization

• The Molecular Architecture of the Nuclear Basket

In-cell cryo-electron microscopy

• Cryo-Focused Ion Beam Milling of Cells